Demand more for your qPCR Assays!

Probe combined with 2 PCR primers for use in the 5’ Nuclease Assay for Real-Time PCR.

• FAM™/Iowa Black® FQ dual-labeled probe
• 100% QC
• Delivered in a single tube for ultimate convenience in 3-5 days
• Optimized for universal thermal cycling use; ideal for many life science research applications
• 4 nm of PrimeTime™ Probe / 8 nm of each PCR Primer; Up to 8000 assays when resuspended at 200 nM primer/100 nM probe concentrations
• Specification sheet accompanying product contains sequence information
• Order via IDT’s validated Real-Time PCR Design Tool

Real-Time PCR Design Tool

IDT’s on-demand assay design tool has been functionally validated.

Features of the Real-Time PCR design tool include:

• Amplicons designed to reduce detection of genomic DNA
• Customizable thermodynamic parameters
• Incorporates genome & transcript information in primer and probe designs
• Results, including sequences of primers and probes, are available for evaluation before ordering
• Primers and probes are designed to flank intron locations in the genome or immature transcript which allow amplicons to span exon-exon junctions in the mature transcript.