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| Sets | Primer/Probe Sequences (5' > 3') | Quantity |
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| InfA | | | SW InfA | | | SW H1 | | | Rnase P | | | H1N1 | |
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| Each panel consists of four sets which each include two primers and one PrimeTime 5’ hydrolysis dual labeled probe. Positive controls templates are also available as ReadyMade Ultramers for each individual set or as a plasmid with all four endogenous transcripts. | | IDT is the worldwide leader in custom oligo manufacturing and routinely manufactures all oligonucleotides with the highest quality controls. Each oligo undergoes 100% QC by mass spectrometry and all results are provided free of charge to the customer on the IDT website. | |
- InfA: designed for universal detection of type A influenza viruses
- SW InfA: designed to detect all swine influenza A viruses
- SW H1: designed to detect swine H1 influenza
- Rnase P: control set
| | Note: These primer/probe sets may undergo modifications as additional information is obtained. | | Set | Primers and Probes | Sequence (5'>3') | Price |
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| InfA1 | | InfA Forward - 10 nm |
GAC CRA TCC TGT CAC CTC TGA C
| $5.00 | | InfA Reverse - 10 nm |
AGG GCA TTY TGG ACA AAK CGT CTA
| $5.00 | | 5' 6-FAM™ / 3' IB®FQ 100 nm PrimeTime™ DLP | TGC AGT CCT CGC TCA CTG GGC ACG | $170.00 | | 5' 6-FAM™ / 3' IB®FQ 250 nm PrimeTime™ DLP | TGC AGT CCT CGC TCA CTG GGC ACG | $240.00 | | 5' 6-FAM™ / 3' IB®FQ 1 µm PrimeTime™ DLP | TGC AGT CCT CGC TCA CTG GGC ACG | $360.00 |
| | SW InfA2 | | SW InfA Forward - 10 nm |
GCA CGG TCA GCA CTT ATY CTR AG
| $5.00 | | SW InfA Reverse - 10 nm |
GTG RGC TGG GTT TTC ATT TGG TC
| $5.00 | | 5' 6-FAM™ / Int IB®FQ 100 nm PrimeTime™ DLP | CYA CTG CAA GCC CA"T" ACA CAC AAG CAG GCA | $180.00 | | 5' 6-FAM™ / Int IB®FQ 250 nm PrimeTime™ DLP | CYA CTG CAA GCC CA"T" ACA CAC AAG CAG GCA | $250.00 | | 5' 6-FAM™ / Int IB®FQ 1 µm PrimeTime™ DLP | CYA CTG CAA GCC CA"T" ACA CAC AAG CAG GCA | $380.00 |
| | SW H12 | | SW H1 Forward - 10 nm |
GTG CTA TAA ACA CCA GCC TYC CA
| $5.00 | | SW H1 Reverse - 10 nm |
CGG GAT ATT CCT TAA TCC TGT RGC
| $5.00 | | 5' 6-FAM™ / Int IB®FQ 100 nm PrimeTime™ DLP | CAG AAT ATA CA"T" CCR GTC ACAA TTG GAR AA
| $180.00 | | 5' 6-FAM™ / Int IB®FQ 250 nm PrimeTime™ DLP | CAG AAT ATA CA"T" CCR GTC ACAA TTG GAR AA
| $250.00 | | 5' 6-FAM™ / Int IB®FQ 1 µm PrimeTime™ DLP | CAG AAT ATA CA"T" CCR GTC ACAA TTG GAR AA
| $380.00 |
| | Rnase P1 | | RNase P Forward - 10 nm | AGA TTT GGA CCT GCG AGC G
| $5.00 | | RNase P Reverse - 10 nm |
GAG CGG CTG TCT CCA CAA GT
| $5.00 | | 5' 6-FAM™ / 3' IB®FQ 100 nm PrimeTime™ DLP | TTC TGA CCT GAA GGC TCT GCG CG
| $170.00 | | 5' 6-FAM™ / 3' IB®FQ 250 nm PrimeTime™ DLP | TTC TGA CCT GAA GGC TCT GCG CG
| $240.00 | | 5' 6-FAM™ / 3' IB®FQ 1 µm PrimeTime™ DLP | TTC TGA CCT GAA GGC TCT GCG CG
| $360.00 |
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| 1 Probes are labeled at the 5’-end with the reporter molecular 6-carboxyfluorescene (FAM) and at the 3’-end with the Iowa Black™ (IBFQ).
2 Probes are labeled at the 5’-end with the reporter molecule 6-carboxyfluorescein (FAM), quenched internally at a modified “T” residue with IBFQ, and modified at the 3’-end to prevent probe extension by Taq polymerase. |
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| IDT offers qPCR positive controls for each of the H1N1 primer/probe sets as well as a universal plasmid control containing all four cloned amplicons. | |
| The qPCR primer/probe set controls are ReadyMadeTM DNA Ultramers that can be used as copy number standards for each assay. Each Ultramer control contains an additional 14 bases (shown as lower case bases in Figure 1). By increasing the size of the amplicon, it is possible to distinguish a positive result as coming from the control or as a possible viral contaminant by running the product on a gel. Finally, each Ultramer Control also contains SP6 and T7 promoter regions. The promoter regions allow for the generation of in vitro transcribed RNA if an RNA transcript control is desired. Each tube contains 6x1013 molecules. | |
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| The universal plasmid control contains positive control sequences for all four target sequences in the swine flu testing panel. The plasmid also contains restriction sites for digestion which will allow for degradation if amplicon contamination occurs. Similar to the Ultramer controls, the plasmid also contains SP6 and T7 promoter regions for generation of in vitro transcribed RNA. The design of the plasmid can be seen in Figure 2. | |
| | | Sequence | Price |
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| InfA Ultramer™ Positive Control - 100 pmoles |
ATT TAG GTG ACA CTA TAG AAG ACC GAT CCT GTC ACC TCT GAC TTT GGG AAT TTT AGG ATT TGT GTT CAT GTC TGT TTA AAC TCT CTC ACC GTG CCC AGT GAG CGA GGA CTG CAG CGT AGA CGC TTT GTC CAG AAT GCC CTC CCT ATA GTG AGT CGT ATT A
| $30.00 |
| | SW InfA Ultramer™ Positive Control - 100 pmoles |
ATT TAG GTG ACA CTA TAG AAG CAC GGT CAG CAC TTA TCC TGA GAA GCT TCT ACT GCA AGC CCA TAC ACA CAA GCA GGC AAT CTG TTT AAA CTC TTG ACC AAA TGA AAA CCC AGC CCA CCC CTA TAG TGA GTC GTA TTA
| $30.00 |
| | SW H1 Ultramer™ Positive Control - 100 pmoles |
ATT TAG GTG ACA CTA TAG AAG TGC TAT AAA CAC CAG CCT CCC ATT CAG AAT ATA CAT CCA GTC ACA ATT GGA AAA TAG AGT TTA AAC AGA TGC CAC AGG ATT AAG GAA TAT CCC GCC CTA TAG TGA GTC GTA TTA
| $30.00 |
| | RNase P Ultramer™ Positive Control - 100 pmoles | ATT TAG GTG ACA CTA TAG AAA GAT TTG GAC CTG CGA GCG CTA TTC TGA CCT GAA GGC TCT GCG CGT AAT CTG TTT AAA CTC TGC TTA CTT GTG GAG ACA GCC GCT CCC CTA TAG TGA GTC GTA TTA | $30.00 |
| | H1N1 Universal Positive Control Plasmid - 100ng | | $100.00 |
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| Use Restrictions. Research Purposes Only: Oligonucleotides and nucleic acid products (“IDT Products”) are manufactured and sold by IDT for the customer’s research purposes only. Except pursuant to a separate, written agreement with IDT, IDT Products are not sold (and have not been approved) for use in any clinical, diagnostic, or therapeutic applications. Obtaining any license(s) or other approvals necessary to use IDT Products in proprietary applications or in any non-research (e.g., clinical) applications is the customer’s exclusive responsibility. IDT will not be responsible or liable for any losses, costs, expenses, or other forms of liability arising out of the unauthorized or unlicensed use of IDT Products. Purchasers of IDT Products shall indemnify and hold IDT harmless for any and all damages and/ or liability, however characterized, related to the unauthorized or unlicensed use of IDT Products. Under no circumstances shall IDT be liable for any consequential damages resulting from any use (approved or otherwise) of IDT Products. No Resale: The sale or resale of IDT Products by any person other than IDT is strictly prohibited without the express, written consent of IDT. All orders accepted by IDT, and all sales of IDT Products, are made subject to the forgoing use restrictions and the customer’s indemnification of IDT. | |
| IDT manufactures and sells the CDC proscribed H1N1 dual-labeled probes for research purposes only under license from both Life Technologies and Roche Molecular Systems. If applicable, IDT requires that customers comply with both of the following licensing statements. | |
| A license to perform the patented 5' Nuclease Process for research is obtained by the purchase of (i) both Licensed Probe and Authorized 5' Nuclease Core Kit, (ii) a Licensed 5' Nuclease Kit, or (iii) license rights from Applied Biosystems. This product contains Licensed Probe. Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,538,848, 5,723,591, 5,876,930, 6,030,787, 6,258,569, and 5,804,375 (claims 1-12 only). The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. The right to use this product in the 5' Nuclease Process under the applicable claims of US Patents Nos. 5,210,015 and 5,487,972, and corresponding patent claims outside the United States, can be obtained through purchase of an Authorized 5' Nuclease Core Kit. Except under separate license rights available from Applied Biosystems, no right under any other patent claim, or to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, or to sublicense, repackage with other products, or resell in any form, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained from the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. | |
| “THIS PROBE IS MADE AND SOLD PURSUANT TO A LICENSE UNDER U.S. PATENT NOS. 6,214,979, 5,210,015, 5487,972, 7,445,900 AND 5,804,375 (CLAIMS 1-12 ONLY) AND CORRESPONDING CLAIMS IN PATENTS OUTSIDE THE U.S. OWNED BY ROCHE. PURCHASE OF THIS PROBE ENTITLES THE PURCHASER TO USE THE PROBE IN A REACTION MIXTURE COVERED ONLY BY CLAIMS 1-12 OF THE 5,804,375 PATENT AND COUNTERPART CLAIMS OF SAID CLAIMS OUTSIDE THE UNITED STATES OWNED BY ROCHE TO PERFORM ONLY INTERNAL RESEARCH USING THE 5’ NUCLEASE PROCESS IN THE DIAGNOSTIC RESEARCH FIELD. NO LICENSE IS HEREBY GRANTED FOR ANY OTHER FIELD OF USE AND NO LICENSE OR OTHER RIGHTS ARE GRANTED TO INCLUDE THIS PROBE IN ANY ROCHE PATENTED KIT OR TO PROVIDE THE RESULTS OF ANY REACTION OR TEST USING THIS PROBE TO ANY THIRD PARTY, EXPRESSLY OR BY IMPLICATION, BY THE SALE OR PURCHASE OF THIS PRODUCT. NO LICENSE OR OTHER RIGHTS ARE INCLUDED UNDER ANY OTHER ROCHE PATENT DIRECTED TO ANY NUCLEIC ACID AMPLIFICATION OR DETECTION PROCESS, REACTION MIXTURE OR KIT, EXPRESSLY OR BY IMPLICATION, BY THE SALE OR PURCHASE OF THIS PRODUCT. PRACTICE OF ANY OTHER ROCHE PATENTED NUCLEIC ACID AMPLIFICATION OR DETECTION PROCESS, THE INCLUSION OF THIS PROBE IN ANY ROCHE PATENTED KIT OR PROVIDING THE RESULTS OF ANY REACTION OR TEST USING THIS PROBE TO ANY THIRD PARTY REQUIRES A SEPARATE LICENSE. FOR INFORMATION ON PURCHASING SUCH LICENSES IN THE DIAGNOSTICS RELATED FIELDS, PLEASE CONTACT THE LICENSING DEPARTMENT OF ROCHE MOLECULAR SYSTEMS, INC., 4300 HACIENDA DRIVE, PLEASANTON, CALIFORNIA 94588, USA.” |
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