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TriFECTin™ Transfection Reagent

View the new TriFECTin™ Technical Report

Trifectin is a proprietary cationic lipid formulation that has been optimized for delivery of IDT’s Dicer-Substrate siRNAs into a wide variety of cell types with minimal toxicity. It is equally potent for delivery of traditional 21-mer siRNAs and is also effective in delivery other kinds of nucleic acids. In particular, Trifectin has been shown to be effective in delivering antisense oligos (ASOs) of all kinds, including anti-miRNA ASOs.

Benefits of Trifectin include:

  • Easy optimization due to low toxicity and good function across a wide range of RNA/lipid transfection ratios
  • Efficient tranfection permitting use of lower RNA concentrations
  • Functional in a wide range of cells

DescriptionPrice
0.5 mL TriFECTin™$150.00 USD
1 mL TriFECTin™ $225.00 USD
5 x 1 mL TriFECTin™ $1,015.00 USD


Technical Note

Contents and Storage
Handle under sterile conditions. Store at +4°C. Trifectin is guaranteed to be stable for a minimum of 6 months when properly stored. Do not freeze or leave at room temperature for extended periods of time.

To qualitatively assess transfection efficiency, we recommend using the dye-labeled transfection control duplexes. A variety of positive controls are available.

Basic Protocol
Mix Trifectin with the siRNA or other nucleic acid, add to the cells, and incubate. Trifectin has low toxicity and it is not necessary to remove the transfection cocktail before an extended incubation period. Use is compatible with both forward and reverse transfection and in manual or robotic systems.
 
 
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