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| Order TriFECTa™ Library & Kit | | Order TriFECTa™ ReadyMade Control Duplexes | | Download TriFECTa™ RNAi Kits Quick Start Guide | | Download TriFECTa™ User's Manual |
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The TriFECTa™ kit contains three Screening Dicer-substrate (DsiRNA) duplexes targeting a specific gene that are selected from a predesigned collection of duplexes in the DsiRNA RefSeq Library. The DsiRNAs target sites were chosen by a rational design algorithm that integrates both traditional 21-mer siRNA design rules as well as new Dicer-substrate design criteria. |
In addition to three target-specific duplexes, the TriFECTa™ kit contains three control sequences that are needed to perform RNAi experiments, including a TYE 563™-labeled transfection control RNA Duplex, a universal negative control RNA duplex, which is absent in human, mouse, and rat genomes and a positive control DsiRNA duplex, which targets a site in the HPRT (hypoxanthine guanine phosphoribosyltransferase gene that is common between human, mouse, and rat and is prevalidated to give >90% knockdown of HPRT when transfected at 10 nM concentration. |
IDT guarantees that at least two of the three Screening DsiRNA duplexes in the TriFECTa™ kit will give at least 70% knockdown of the target mRNA when used at 10 nM concentration and assayed by quantitative RT-PCR when the fluorescent transfection control duplex indicates that >90% of the cells have been transfected and the HPRT positive control demonstrates 90% knockdown efficiency. |
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- Three target-specific Dicer-Substrate siRNA duplexes (2 nmoles each)
- Fluorescent-labeled transfection control duplex: TYE 563™ (1 nmole)
- HPRT-S1 DS Positive Control duplex (1 nmole)
- NC1 Negative Control, universal negative control duplex (1 nmole)
- Nuclease-Free Duplex Buffer (100mM KAc/30 mM HEPES pH 7.5)
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