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miRFire Nucleic Acid Labeling System

miRNA StarFire® is a proprietary labeling system for generating radiolabeled oligo probes with 10-fold greater specific activity than traditional 5'-end labeling with polynucleotide kinase. It is based on 3'-end labeling with DNA polymerase. This labeling method is particulary useful for probes to identify small regulatory RNA and analyze the expression of microRNA genes.1-3 For more information, please see our tech bulletin by clicking here.

miRNA StarFire® Complete Kit
Sufficient for 25 labeling reactions

Includes:
Exo-Klenow DNA Polymerase
StarFire® 10X Buffer Mix
StarFire® Stop Buffer
One PAGE-purified StarFire®
­­Custom Probe (up to 50 bases)
StarFire® Universal Template
miRNA StarFire® Universal­Template­ ­
Sufficient for 300 labeling reactions
0.5 OD (in single tube)
HPLC Purified
Quality Control checked by mass spectrometry and CE


miRNA StarFire® Custom Probes
(up to 50 bases)
Desalted Only
 / base
PAGE Purified*
 / base + StarFire® PAGE,


miRNA StarFire® Refill Kit
Sufficient for 25 labeling reactions
Exo-Klenow DNA Polymerase
StarFire® 10X Buffer Mix
StarFire® Stop Buffer
StarFire® Universal Template



Reference

1. Use of high specific activity StarFire® oligonucleotide probes to visualize low-abundance pre-mRNA splicing intermediates in S. pombe. Behlke, M.A., Dames, S.A., McDonald, W.H., Gould, K.L., Devor, E.J., and Walder, J.A., BioTechniques, 29:892-897 (2000).

2. Temporal regulation of microRNA expression in Drosophila melanogaster mediated by hormonal signals and Broad-Complex gene activity. Sempere, L.F., Sokol, N.S., Dubrovsky, E.B., Berger, E.M., Ambros, V., Development Biology 259: 9 (2003).

3. The Expression of the let-7 Small Regulatory RNA Is Controlled by Ecdysone during metamorphosis in Drosophila melanogaster. Sempere, L.F., Dubrovskaya, V.A., Dubrovsky, E.B., Berger, E.M., Ambros, V., Development Biology, vol. 244: 170 (2002).


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