Mass Spectrometry and CE -
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1. Log into the IDT Ordering System.
2. Go to Past Order Status.
3. Select the order containing the purified oligos whose Mass Spectrometry or CE you want to view.
4. To view the Mass Spectrometry or CE trace of a particular item, click the Mass Spectrometry or CE button next to the item.

Mass Spectrometry

All oligos synthesized at IDT are analyzed by mass spectrometry. Matrix-Assisted Laser Desorption/Ionization-Time Of Flight (MALDI-TOF) mass spectrometry is suitable for use in a high-throughput QC setting and can resolve oligonucleotides up to 50 bases in length (~15,000 Daltons). Electrospray Ionization (ESI) mass spectrometry can resolve longer oligonucleotides (up to ~150 bases in length).

Mass Spectrometry Analysis

Capillary Electrophoresis

CE provides quantitative analysis of micropurity. Nanoliter samples of oligonucleotides are electrokinetically injected into a fused silica capillary and filled with a denaturing gel under an electric potential. The oligonucleotides move through the gel matrix and are separated according to length. Absorbance at 260nm is detected as the oligo passes through a window in the capillary. The resulting electropherogram provides the percent purity of the full-length product as well as truncated products.

CE Analysis

Analytical HPLC

HPLC is a universally accepted method of sample quality assessment. IDT employees use both Reverse-Phase and Ion-Exchange methods to assess product purity. These methods are employed on purified oligos where the composition is incompatible with CE technology.

HPLC Analysis

For more information on mass spectrometry or CE traces, please contact Technical Support by phone at 1-800-328-2661 or by email at techsupport@idtdna.com.

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