Extinction Coefficients
Measured and calculated extinction coefficients at 260 nm and neutral pH for single-stranded RNA (A260 units/µmol)
| RNA |
e260 |
RNA |
e260 |
| pA |
15.4 |
CpG |
8.9 |
| pC |
7.2 |
CpU |
8.1 |
| pG |
11.5 |
GpA |
12.6 |
| pU |
9.9 |
GpC |
8.7 |
| ApA |
13.7 |
GpG |
10.8 |
| ApC |
10.5 |
GpU |
10.6 |
| ApG |
12.5 |
UpA |
12.3 |
| ApU |
12.0 |
UpC |
8.6 |
| CpA |
10.5 |
UpG |
10.0 |
| CpC |
7.1 |
UpU |
9.8 |
Approximate extinction coefficients (accuracy about 10%) for
single-stranded RNA and 2´-O-methylRNA m residues long of
sequence DpEpFpGp----KpL can be calculated from the nearest
neighbour formula:
eDpEpFpGp-----KpL = 2(eDpE +
eEpF + eFpG + ----- +eKpL) -
(eE + eF + eG ---- + eK) | 
