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Integrated DNA
Technologies, BVBA
Provisorium 2
Minderbroedersstraat 17-19
B-3000 Leuven
BELGIUM
Tel: +32-16-337096
Fax: +32-16-337097
info@rna-tec.com
How to reach us
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Oligonucleotides
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Analytical Anion Exchange HPLC chromatogram: To confirm levels of purity from the standard 95 % to the 99 %, each oligonucleotide is delivered with an analytical anion-exchange HPLC chromatogram.
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As an example of the QC for oligos with an standard purity, see below a analytical Anion Exchange HPLC of a 45 mer of RNA.
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Analytical Reversed Phase HPLC chromatogram: For oligonucleotides to be used in structural studies, for example X-ray crystallography or NMR spectroscopy, higher purities available on request (up to 99 %). The samples are delivered with an extra chromatogram of an analytical Reversed Phase HPLC.
As an example of the high quality and purity, see below a chromatogram of an analytical Reversed Phase HPLC of a 29 mer of RNA on a 10 µmol scale.
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Mass spectrometry: As an important and absolute check on the authenticity of the product a final analysis by mass spectrometry is included to verify that the molecular ion corresponds to what is expected.
| For an example of a MS spectra supplied with each oligonucleotide click pdf-logo.
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| This is an electrospray mass spectrum of an oligonucleotide where all 18
internucleotide linkages were sulfurized. The spectrum shows a main peak
corresponding to a mass of 6680.8 (the expected mass of the oligo). The
other (small) peak corresponding to a mass of 6664,6 represents the full
length oligo with 17 phosphorothioates and one phosphodiester linkage. From
the ratio of these peaks and based on the length of the oligo we can
calculate that the average sulfurization efficiency is 99.6 %. Also, notice
that there are no (n-1) residu 's in the sample.
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Characteristics of the oligonucleotide: Each oligonucleotide is delivered with its characteristics: molecular weight, extinction coefficient, nmole / OD260 and µg / OD260.
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Custom synthesis
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Custom synthesis products are delivered with all necessary analytical data.
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