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Oligo Design   Quick Start Guide   ddRNAi (shRNA) System: Promega GeneClip U1 Hairpin Cloning System Promega siSTRIKE U6 Hairpin Cloning System Promega siLentGene U6 Cassette RNA Interference System Promega siLentGene-2 U6 Hairpin Cloning System Promega T7 RiboMax Express RNAi System: siRNA Promega T7 RiboMax Express RNAi System: Hairpin siRNA Custom ddRNAi System 5' End Site 5' Control Site Loop Sequence 3' Control Site 3' End Site None 5' GeneClip 3' GeneClip 5' siStrike 3' siStrike UU Overhang AatI AatII AccII AccIII Acc16I Acc36I Acc65I AccBSI AceIII AciI AclI AclWI AcuI AcvI AfaI AfeI AflII AgeI AhaIII AhlI AluI AlwI Alw26I Alw44I Aor51HI ApaI ApaLI AscI AseI AsiAI AsiSI Asp718I AspA2I AspCNI AspLEI AssI AsuII AsuC2I AsuHPI AsuNHI AvaIII AviII AvrII BalI BamHI BanIII BauI BbeI Bbr7I BbrPI BbsI BbuI BbvI BbvII BbvCI BccI Bce83I BceAI BcefI BciVI BclI BcuI BfaI BfiI BfrI BfrBI BfuI BfuAI BfuCI BglII BinI BlfI BlnI BmgBI BmrI BmtI BpiI BpmI Bpu14I BpuAI BpuEI BsaI Bsa29I BsaMI BsbI BscAI BscGI Bse1I BseAI BseCI Bse3DI BseGI BseMI BseMII BseNI BsePI BseRI BseXI BseX3I BseYI BsgI Bsh1236I BshFI BshTI BsiI BsiSI BsiWI BslFI BsmI BsmAI BsmBI BsmFI Bso31I BsoMAI Bsp13I Bsp19I Bsp68I Bsp106I Bsp119I Bsp120I Bsp143I Bsp1407I BspANI BspCI BspCNI BspDI BspEI BspGI BspHI BspLU11I BspMI BspMII BspMAI BspNCI BspPI BspTI BspT104I BspTNI BspXI BsrI BsrBI BsrDI BsrGI BsrSI BssHI BssHII BssNAI BssSI Bst6I Bst98I Bst1107I BstAUI BstBI Bst2BI BstF5I BstFNI BstH2I BstHHI BstKTI BstMBI BstSNI BstUI BstV1I BstV2I BstZI BstZ17I Bsu15I BsuRI BsuTUI BtgZI BtrI BtsI BveI CciNI CdiI CfoI Cfr9I Cfr42I ChaI ClaI Csp6I Csp45I CspAI CstMI CviAII CviRI DpnI DpnII DraI DrdII EagI Eam1104I EarI EciI Ecl136II EclXI Eco31I Eco32I Eco47III Eco52I Eco57I Eco72I Eco105I Eco147I EcoICRI EcoRI EcoRV EcoT22I EgeI EheI EsaBC3I Esp3I FaqI FatI FauI FauNDI FbaI FinI FnuDII FokI FseI FspI FspBI FunI FunII GdiII GsuI HaeIII HapII HgaI HhaI Hin1II Hin4II Hin6I HinP1I HindIII HpaI HpaII HphI HpyAV HpyCH4IV HpyCH4V Hsp92II HspAI KasI KpnI Kpn2I KspI Ksp22I Ksp632I KspAI Kzo9I LweI MaeI MaeII MbiI MboI MboII MfeI MlsI MluI MluNI MlyI Mly113I MnlI Mph1103I MroI MroNI MscI MseI MspI Msp20I MspCI MssI MstI MunI Mva1269I MvnI MvrI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsbI NsiI NspV PacI PaeI PaeR7I PagI PalI PauI PceI PciI PctI PdiI Pfl23II Pfl1108I PhoI PinAI PleI Ple19I PmaCI PmeI PmlI PpsI Ppu10I PshBI PsiI Psp1406I PspAI Psp124BI PspCI PspLI PspOMI PstI PvuI PvuII RcaI RleAI RsaI RsrII Rsr2I SacI SacII SalI SapI Sau3AI SbfI ScaI SchI SciI SdaI SelI SfaNI SfoI Sfr274I Sfr303I SfuI SgfI SgrBI SimI SlaI SmaI SmiI SmuI SnaI SnaBI SpaHI SpeI SphI SplI SrfI Sse9I Sse232I Sse8387I SseBI SsiI SspI SspBI SspD5I SstI Sth132I StrI StsI StuI SunI SwaI TaiI TaqI TaqII TaqII TasI TauI TliI Tru1I Tru9I TscI Tsp509I TspDTI TspEI TspGWI Tth111II UbaF5I UbaF6I UbaPI UthSI Vha464I VneI VspI XbaI XceI XhoI XmaI XmaIII XmaCI XmaJI XspI ZhoI ZraI ZrmI Zsp2I None U6 Cassette T7 Promoter Terminator U6 Terminator U6 Term Comp None TTCAAGAGA AAGTTCTCT TTTGTGTAG CTTCCTGTCA None U6 Cassette T7 Promoter Terminator U6 Terminator U6 Term Comp None 5' GeneClip 3' GeneClip 5' siStrike 3' siStrike UU Overhang AatI AatII AccII AccIII Acc16I Acc36I Acc65I AccBSI AceIII AciI AclI AclWI AcuI AcvI AfaI AfeI AflII AgeI AhaIII AhlI AluI AlwI Alw26I Alw44I Aor51HI ApaI ApaLI AscI AseI AsiAI AsiSI Asp718I AspA2I AspCNI AspLEI AssI AsuII AsuC2I AsuHPI AsuNHI AvaIII AviII AvrII BalI BamHI BanIII BauI BbeI Bbr7I BbrPI BbsI BbuI BbvI BbvII BbvCI BccI Bce83I BceAI BcefI BciVI BclI BcuI BfaI BfiI BfrI BfrBI BfuI BfuAI BfuCI BglII BinI BlfI BlnI BmgBI BmrI BmtI BpiI BpmI Bpu14I BpuAI BpuEI BsaI Bsa29I BsaMI BsbI BscAI BscGI Bse1I BseAI BseCI Bse3DI BseGI BseMI BseMII BseNI BsePI BseRI BseXI BseX3I BseYI BsgI Bsh1236I BshFI BshTI BsiI BsiSI BsiWI BslFI BsmI BsmAI BsmBI BsmFI Bso31I BsoMAI Bsp13I Bsp19I Bsp68I Bsp106I Bsp119I Bsp120I Bsp143I Bsp1407I BspANI BspCI BspCNI BspDI BspEI BspGI BspHI BspLU11I BspMI BspMII BspMAI BspNCI BspPI BspTI BspT104I BspTNI BspXI BsrI BsrBI BsrDI BsrGI BsrSI BssHI BssHII BssNAI BssSI Bst6I Bst98I Bst1107I BstAUI BstBI Bst2BI BstF5I BstFNI BstH2I BstHHI BstKTI BstMBI BstSNI BstUI BstV1I BstV2I BstZI BstZ17I Bsu15I BsuRI BsuTUI BtgZI BtrI BtsI BveI CciNI CdiI CfoI Cfr9I Cfr42I ChaI ClaI Csp6I Csp45I CspAI CstMI CviAII CviRI DpnI DpnII DraI DrdII EagI Eam1104I EarI EciI Ecl136II EclXI Eco31I Eco32I Eco47III Eco52I Eco57I Eco72I Eco105I Eco147I EcoICRI EcoRI EcoRV EcoT22I EgeI EheI EsaBC3I Esp3I FaqI FatI FauI FauNDI FbaI FinI FnuDII FokI FseI FspI FspBI FunI FunII GdiII GsuI HaeIII HapII HgaI HhaI Hin1II Hin4II Hin6I HinP1I HindIII HpaI HpaII HphI HpyAV HpyCH4IV HpyCH4V Hsp92II HspAI KasI KpnI Kpn2I KspI Ksp22I Ksp632I KspAI Kzo9I LweI MaeI MaeII MbiI MboI MboII MfeI MlsI MluI MluNI MlyI Mly113I MnlI Mph1103I MroI MroNI MscI MseI MspI Msp20I MspCI MssI MstI MunI Mva1269I MvnI MvrI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsbI NsiI NspV PacI PaeI PaeR7I PagI PalI PauI PceI PciI PctI PdiI Pfl23II Pfl1108I PhoI PinAI PleI Ple19I PmaCI PmeI PmlI PpsI Ppu10I PshBI PsiI Psp1406I PspAI Psp124BI PspCI PspLI PspOMI PstI PvuI PvuII RcaI RleAI RsaI RsrII Rsr2I SacI SacII SalI SapI Sau3AI SbfI ScaI SchI SciI SdaI SelI SfaNI SfoI Sfr274I Sfr303I SfuI SgfI SgrBI SimI SlaI SmaI SmiI SmuI SnaI SnaBI SpaHI SpeI SphI SplI SrfI Sse9I Sse232I Sse8387I SseBI SsiI SspI SspBI SspD5I SstI Sth132I StrI StsI StuI SunI SwaI TaiI TaqI TaqII TaqII TasI TauI TliI Tru1I Tru9I TscI Tsp509I TspDTI TspEI TspGWI Tth111II UbaF5I UbaF6I UbaPI UthSI Vha464I VneI VspI XbaI XceI XhoI XmaI XmaIII XmaCI XmaJI XspI ZhoI ZraI ZrmI Zsp2I 5' TCTC  NNN...NNN CTTCCTGTCA NNN...NNN  CT     3'        ||||||||| |||||||||| |||||||||  ||      3'       NNN...NNN GAAGGACAGT NNN...NNN  GACGTC 5' Use Cassette System Target Length: (17-33 Bases) Single Strand DNA Product Custom Loop Sequence: Add Loop Reverse Target-Loop Orientation Next References: Lee, N. et al. (2002) Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells. Nat. Biotechnol. 20, 500-5.