Integrated DNA Technologies

Oligo Design

ddRNAi (shRNA) System:

5' End Site	5' Control Site	Loop Sequence	3' Control Site	3' End Site

5' TCTC NNN...NNN CTTCCTGTCA NNN...NNN CT 3'

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3' NNN...NNN GAAGGACAGT NNN...NNN GACGTC 5'

Use Cassette System

Target Length: (17-33 Bases)

Single Strand DNA Product

Custom Loop Sequence:

Add Loop

Reverse Target-Loop Orientation

References:
Lee, N. et al. (2002) Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells. Nat. Biotechnol. 20, 500-5.