Purification is recommended for all oligos greater than 40 bases in length and for many modified oligos. For demanding applications such as multiplex PCR, cloning, mutagenesis or antisense/RNAi methods, additional purification will significantly improve oligonucleotide performance. Every purified oligo up to 60 bases in length receives QC by capillary electrophoresis (CE) to document final purity. PAGE obtains extremely high purity, especially for unmodified oligos and UltramersTM. PAGE purification is strongly recommended for oligos over 60 bases in length. For unmodified oligos, purity of >90% is routinely achieved. Purity may vary due to oligo length, modifications, or sequence composition. HPLC is well suited to purify any modified oligo or unmodified sequences up to 60 bases in length. Purity of > 85% is guaranteed. Purity may vary due to oligo length, modifications, or sequence composition. Ion-exchange HPLC (IE-HPLC) is available to purify long DNA oligos. For unmodified oligos, purity of > 85% is guaranteed. Purity may vary due to oligo length, modifications, or sequence composition. For the most demanding applications, IDT offers Dual HPLC Purification and Dual PAGE and HPLC Purification. These methods result in oligos of the highest possible purity. Dual HPLC purification is used to purify Dual-Labeled Probes and Molecular Beacons with NHS Ester modifications. Purity may vary due to oligo length, modifications, or sequence composition. |